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Atherosclerosis: Experimental Methods and Protocols (Methods in Molecular Medicine)
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Atherosclerosis: Experimental Methods and Protocols (Methods in Molecular Medicine) Description
In Atherosclerosis: Experimental Methods and Protocols, Angela Drew and a panel of experts have assembled a comprehensive collection of traditional and cutting-edge techniques for investigating this disease and its possible treatments. Each readily reproducible method includes step-by-step instructions and practical information regarding sample collection, the choice of animal model system, experimental design, and practical data analysis techniques. Comprehensive and richly detailed, Atherosclerosis: Experimental Methods and Protocols enables all biomedical investigators to select those optimized techniques that may be most fruitfully used to study the development, progression, and treatment of atherosclerotic lesions today.
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1 of 1 people found the following review helpful.
A Must Have Resource For Vasular Research
By S. Shrader
This book is a necessary resource for anyone in the field of vascular pathology, medical device pathology, or vascular research in general. Whether you are a study sponsor, pathologist, study director, scientist, research associate/assistant or laboratory manager/tech, you should familiarize yourself with this book. It allows the reader to make a better-informed decision regarding the type of research model appropriate to the type of research being performed. It also describes invaluable techniques (e.g. proper excision of treated arteries/veins, tissue processing, embedding techniques, and special stains). The contributors are all experts in atherosclerotic research and there are many helpful references in the back of the book. For those who do not like blindly paying a hundred dollars for a book, here are the chapters:
1. Animal Models of Diet-Induced Atherosclerosis
2. Mechanical Injury Models
3. Genetically Manipulated Models of Atherosclerosis in Mice
4. Lipoprotein Isolation and Analysis from Serum by Preparative
Ultracentrifugation
5. Separation of Plasma Lipoproteins in Self-Generated Gradients of
Iodixanol
6. Fractionation of Lipoprotein Subclasses in Self-Generated Gradients of
Iodixanol
7. Detecting Cholesteryl Ester Transfer Protein in Plasma
8. Determination of Plasma Homocysteine
9. Assay for Serum Glycated Lipoproteins
10. Lipoprotein(a) Quantitation
11. Isolation and Characterization of Glycosaminoglycans from Human
Atheromatous Vessels
12. Culture of Human Smooth Muscle Cells
13. Culture of Human Endothelial Cells
14. Assay for Cell Migration and Invasion of Vascular Smooth Muscle Cells
15. Collection and Processing of Arterial Specimens for Histological
Analysis
16. Intravascular Ultrasound Imaging in the Quantitation of
Atherosclerotic Tissue
17. Identification of Cell Types and Quantification of Lesion Composition
18. Nonradioactive In Situ Hybridization of Atherosclerotic Tissue
19. Evaluation of Angiogenesis
20. Evaluation of Cell Proliferation in Human Atherosclerotic Lesions
21. Gene Transfer to the Vasculature
22. A Pig Model of Vein Graft Disease
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